LOCUS       pLV-[hsa-let-7i]        9138 bp    DNA     circular     15-MAR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7059..7919
                     /label=Amp(R)
     promoter        8120..8660
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5664..5898
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6473..6928)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5970..6100
                     /label=SV40 pA
     misc_feature    6101..6314
                     /label=SV40 ori
     misc_feature    4985..5581
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3603
                     /label=intron
     CDS             3650..4975
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3393
                     /label=hsa-let-7i precursor
BASE COUNT     2134 a      2390 c      2514 g      2100 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca ggggcggcgg 
     3121 gccgacgtcg gcggcgagga aggacggagg agcgccggag ccgctttgct gccctccgcg 
     3181 tggtcccgtg cctccccgac accatggccc tggctgaggt agtagtttgt gctgttggtc 
     3241 gggttgtgac attgcccgct gtggagataa ctgcgcaagc tactgccttg ctagtgctgg 
     3301 tgatgctcag cgccgcggag gacaatggct gggaatcccc tttgttttcc ggggcgctgc 
     3361 ctggggacag cccgcgaagc ctcggcgccc ggcgctagct cgagcttttg gagtacgtcg 
     3421 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 
     3481 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 
     3541 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 
     3601 caggtgtcgt gaaaactacc cctctagagt cgagctaccg gtcgccacca tggtgagcaa 
     3661 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga 
     3721 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga 
     3781 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga 
     3841 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat 
     3901 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt 
     3961 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat 
     4021 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa 
     4081 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg 
     4141 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac 
     4201 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt 
     4261 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg 
     4321 ccgccactcc accggcggca tggacgagct gtacaaggac ccaccggtcg ccaccatgac 
     4381 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 
     4441 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 
     4501 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 
     4561 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 
     4621 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 
     4681 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 
     4741 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 
     4801 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 
     4861 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 
     4921 cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg cctgagcggc 
     4981 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 
     5041 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 
     5101 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 
     5161 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 
     5221 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 
     5281 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 
     5341 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 
     5401 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 
     5461 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 
     5521 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 
     5581 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 
     5641 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 
     5701 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 
     5761 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 
     5821 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 
     5881 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 
     5941 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 
     6001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     6061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 
     6121 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     6181 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 
     6241 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg 
     6301 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 
     6361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 
     6421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 
     6481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 
     6541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 
     6601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 
     6661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 
     6721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 
     6781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 
     6841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 
     6901 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 
     6961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 
     7021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 
     7081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 
     7141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 
     7201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 
     7261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 
     7321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 
     7381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 
     7441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 
     7501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 
     7561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 
     7621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 
     7681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 
     7741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 
     7801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 
     7861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 
     7921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 
     7981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 
     8041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 
     8101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 
     8161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 
     8221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 
     8281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 
     8341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 
     8401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 
     8461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 
     8521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 
     8581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 
     8641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 
     8701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 
     8761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 
     8821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 
     8881 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 
     8941 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 
     9001 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 
     9061 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg 
     9121 aacaaaagct ggagctgc 
//
